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You Searched For: Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Product Image-102877-586

SPLINTR® Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: SplintR Ligase, also known as PBCV-1 DNA Ligase or Chorella virus DNA Ligase, efficiently catalyzes the ligation of adjacent, single-stranded DNA olig...

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Product Image-102969-106

NEBNext Ultra II Ligation Module, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: The NEBNext Ultra II Ligation Module has been optimized to efficiently ligate DNA adaptors compatible with Illumina® sequencing to end-repaired, dA-ta...

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Product Image-PAM8221

LigaFast Rapid DNA Ligation System, Promega

Supplier: Promega Corporation
Description: T4 DNA Ligase and buffer combination for rapid (5-minute) ligations.

Certificates

Vendor Image-PAG6050

HaloTag Cloning Starter System, 1 each, Promega

Catalog Number: (PAG6050)
Supplier: Promega Corporation
Description: HaloTag Cloning Starter Kit: designed to use Flexi Cloning System to clone protein coding sequences into mammalian HaloTag expression vectors; clone a...
Product Image-102715-904

Thermostable 5' AppDNA/RNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Thermostable 5´ App DNA/RNA Ligase is a point mutant of catalytic lysine of RNA ligase from Methanobacterium thermoautotrophicum

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Product Image-76538-670

NEBridge Ligase Master Mix, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: NEBridge Ligase Master Mix is a 3X master mix for Golden Gate Assembly. Designed for use with NEB Type IIS restriction enzymes, this master mix contai...

Product Image-101640-958

T4 RNA Ligase 2 Truncated, K227Q, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 2, truncated K227Q (T4 Rnl2tr K227Q) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.

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Product Image-76717-036

EnGen® Spy Cas9 HF1

Supplier: New England Biolabs (NEB)
Description: EnGen® Spy Cas9 HF1 is a high-fidelity, quadruple substitution (N497A/R661A/Q695A/Q926A) variant of EnGen Spy Cas9 NLS from streptococcus pyogenes wit...

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Product Image-101386-088

T4 RNA Ligase 2, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity

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Product Image-PAC8820

Flexi System, Transfer, 100 transfer reactions, Promega

Catalog Number: (PAC8820)
Supplier: Promega Corporation
Description: The Flexi Vector System provides a rapid, efficient, high-fidelity way to transfer protein-coding regions without the need to resequence.

Certificates


Product Image-PAM1804

T4 DNA Ligase, Promega

Supplier: Promega Corporation
Description: For ligation of DNA ends, mutagenesis and cDNA cloning.

Certificates

Product Image-PAC1263

T4 DNA Ligase 10X Buffer, Promega

Catalog Number: (PAC1263)
Supplier: Promega Corporation
Description: For ligation of DNA ends.

Certificates


Product Image-76407-348

Salt-T4 DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hyd...

Product Image-102877-802

NEBUILDER HiFi DNA Assembly® Master Mix, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: NEBuilder HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly.

SDS Certificates Small Business Enterprise

Product Image-101228-182

T4 DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.

SDS Certificates Small Business Enterprise

Product Image-102877-808

NEBUILDER HiFi DNA Assembly® Cloning Kit, New England Biolabs

Catalog Number: (102877-808)
Supplier: New England Biolabs (NEB)
Description: The NEBuilder HiFi DNA Assembly Cloning Kit combines the power of the NEBuilder HiFi DNA Assembly Master Mix with NEB 5-alpha Competent E

Small Business Enterprise


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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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